FIG. 6.

S. cerevisiae SLA2 and C. albicans SLA2 have a talin-like domain which is not required for Sla2p function in S. cerevisiae. (A) Comparison of ScSla2p, CaSla2p, and mouse talin domains. Amino acid positions of the domains illustrated are noted below the genes. Percent identity between domains of the proteins is indicated. Pro, proline-rich region; Q and L, glutamine- and leucine-rich regions within the long coil domain. Only the talin domain of murine talin has similarity to the Sla2 proteins. (B) Deletion analysis of ScSLA2 domains required for INT1-induced filamentous growth. Illustration of deletion alleles obtained from Reizman and colleagues (not underscored) and Drubin and colleagues (underscored). IIFG, INT1-induced filamentous growth determined using the scoring system described in Table 2.