TABLE 2.

Some actin alleles affect INT1-induced filamentous growth

Actin allelea	Amino acids changed to alanineb	Actin subdomain mutatedc	INT1-induced filamentous growthd	Filamentous colony growthe	Bud site selection patternf	Synthetic lethality withg
ACT1			+++	+++	Unipolar
Temperature sensitivity
act1-101	363, 364	1	+++			sla2Δ
act1-113	210, 211	4	+++	+++	Unipolar	sac6Δ
act1-119	116, 117, 118	1	+++			sac6Δ
act1-120	99, 100	1	++	−	Random	sla2Δ
act1-122	80, 81	2	+++
act1-124	56, 57	2	+	−	Random	sla2Δ, sac6Δ
act1-129	177, 179	3	−	−	Random	sla2Δ, sac6Δ, abp1Δ
act1-133	24, 25	1	+++			sla1Δ, sla2Δ
Pseudo-wild type
act1-104	315, 316	3	++	+	Unipolar
act1-115	195, 196	4	+++			sla2Δ, sac6Δ
act1-117	183, 184	4	+++	++	Bipolar
act1-123	68, 72	2	+++

^aStrains used were YJB2603 to YJB2618, as listed in Table 1. 

^bPhenotypes are as described by Wertman et al. (67). 

^cDomains are as assigned by Botstein et al. (11). 

^dProportion of cells forming elongated filamentous cells. +++, like wild type; ++, 25 to 75% of wild-type levels; +, 5 to 25% of wild-type levels; −, less than 2% of wild-type levels. 

^eFilamentous growth was described by Cali et al. (14). +++, like wild type; ++, 80 to 100% of colonies had filaments, but filaments were disorganized relative to the wild type; +, 1 to 10% of colonies had filaments; −, no filaments extending beyond the perimeter of the colony. 

^fBud site selection was determined by Cali et al. (14). 

^gData were reviewed by Botstein et al. (11).