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. 2023 Feb 24;14(2):157. doi: 10.1038/s41419-023-05684-5

Fig. 5. MXRA5 promotes epithelial-to-mesenchymal cell transition (EMT) in pancreatic cancer cells.

Fig. 5

Scatter plots of correlation between MXRA5 mRNA expression and EMT marker genes from the TCGA-PAAD database (A). Ridge plot shows the correlation between MXRA5 mRNA expression and ECM signature markers in pancreatic cancer (B). Puromycin-selected priPC-1 primary human pancreatic cancer cells or PANC-1 cells, with the lentiviral MXRA5 overexpression construct (“MXRA5-OE”), the corresponding vector (“Vec”), MXRA5-sh-S1 (“MXRA5-sh”), the scramble control shRNA (“c-sh”), the sgRNA-CRISPR/dCas-9 MXRA5-KO lentiviral construct (“MXRA5-ko”) or Cas9 control construct (“Cas9-C”), were cultured and expression of EMT marker proteins was tested (C and E). The characteristic EMT morphology in MARA5-overexpressed priPC-1 primary cancer cells were presented (D). Error bars stand for mean ± standard deviation (SD, n = 5). “TPM” stands for transcripts per million. “ECM” stands for extracellular matrix. *P < 0.05 versus “Vec” cells. Experiments in this figure were repeated five times. Scale bar = 100 μm.