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. 2023 Feb 24;14:1055. doi: 10.1038/s41467-023-36691-x

Fig. 6. LAIR2 expression in CD4+FOXP3+ T cells is associated with HNSCC progression.

Fig. 6

a A t-SNE plot shows the T/NK cell clusters including naïve T (IL7R+), CD8+CCL5+, Treg (CD4+FOXP3+), CD4+CD154+, cycling T, and NK cells (top). ND, non-determined. Proliferation scores and the number of cells across T cell clusters are shown (bottom). b Boxplots show the proportion of the T cell clusters according to the tissue types and HPV infection status. Statistical significance of the two-sided t-tests is indicated. c Distributions of the pseudo-times in each T cell cluster are shown according to the tissue types and HPV infection status. d A t-SNE plot shows the LAIR2 expression in the Tregs (left). Stepwise expression of LAIR2 in Tregs across the tissue types is shown (right). e Correlation plots show the COL1A1 expression levels in fibroblasts with the LAIR2 expression levels in Tregs (left) or the proportion of Tregs (right) across the tissue types. 95% confidence interval (CI) is indicated with gray color. f FOXP3 expression levels are measured by RT-PCR in the Tregs (see “Methods”) treated with or without collagen I (10 µg/mL), respectively. n = 3 biologically independent experiments. Data are shown as mean ± SD. g Representative FACS analysis shows the percentages of the FOXP3+CD25+Tregs in PBMCs (left). A dot plot shows the percentages of FOXP3+CD25+Tregs in the PBMCs treated with phosphate-buffered saline (PBS) or collagen I, respectively (right). P = 0.0194. For each group, PBMCs from five patients were evaluated. h Representative FACS analysis shows the LAIR2 +FOXP3+Tregs in the PBMCs treated with PBS or collagen, respectively (left). A dot plot shows the percentages of LAIR2+FOXP3+CD25+Tregs in the PBMCs treated with PBS or collagen I, respectively (right). P = 0.0298. In g, h, for each group, PBMCs from five patients were evaluated. *,P  <  0.05; **,P  <  0.01; ***,P  <  0.001; ns, nonsignificant by the Student two-tailed unpaired t-test. In a, b, d, box plots show the median (center line), the upper and lower quantiles (box), and the range of the data (whiskers). Source data are provided as a Source Data file.