Plate format	Cell line
	CHO-K1	HEK293-T
96-well (cAMP measurement)	2·104/w	2.5·104/w
48-well (plasma membrane expression)	0.8·105/w	1.2·105/w
6-well (total expression)	5·105/w	8·105/w

Seed the cells in a total volume of 200 μL/w in 96-well plates, 500 μL/w in 48-well plates and 1 mL/w in 6-well plates.