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. 2023 Feb 14;4(1):102120. doi: 10.1016/j.xpro.2023.102120
PCR cycling conditions
Steps Temperature Time Cycles
Initial Denaturation 98°C 30 s 1
Denaturation 98°C 20 s 35
Annealing 65°C 30 s
Extension 72°C 90 s
Final extension 72°C 5 min 1
Hold 10°C forever