Versene buffer

Reagent	Final concentration	Amount
EDTA 0.5 M, pH 8	0.5 mM	0.5 mL
D-PBS 1×	N/A	500 mL
Total	N/A	500 mL

Store in aliquots at 4°C for no longer than 6 months. Do not adjust final pH. It is about 7.3.