Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
HBSS/ HEPES assay buffer
| Reagent | Final concentration | Amount |
|---|---|---|
| 10× HBSS buffer | 1× | 100 mL |
| HEPES (1 M) | 20 mM | 20 mL |
| ddH2O | N/A | Add to 1,000 mL |
| Total | N/A | 1,000 mL |
Adjust pH to 7.4.
Store in aliquots at −20°C to avoid repeated freeze-thaw cycles and contamination. Do not store longer than 24 months.
