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. 2023 Feb 24;14(2):156. doi: 10.1038/s41419-023-05689-0

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — A Gating strategy and the fluorescence minus one (FMO) sample to identify CD11c⁻ and CD11c⁺ microglia on tMCAO 14d. qPCR for CD11c (B), Csf1 (C), Igf-1 (D), Spp1 (E), CD68 (F), and Axl (G) mRNA expression in CD11c⁻ and CD11c⁺ microglia, respectively. n ≥ 3 repeats. The results of qPCR for Abca1 (H), Abcg1 (I), Apoe (J), Apoc1 (K), and Lpl (L) mRNA expression in CD11c⁻ and CD11c⁺ microglia, respectively, on the 7th Day post-stroke. n = 5 repeats. M Representative images of CD11c (red), CD68 (orange), IGF-1 (gray), and MBP (green) immunostaining on the sham group and 7th Day after tMCAO. Scale bars, 100 μm. *p < 0.05, **p < 0.01, and ***p < 0.001. B–L Compared to CD11c^- group. Values were mean ± SEM.