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. 2023 Feb 24;7:22. doi: 10.1038/s41698-023-00363-2

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Table demonstrating the number of annotated well-differentiated and poorly differentiated tumor regions for each patient. b Volcano plot comparison of all glycans in annotated well-differentiated tumor regions (n = 40) and annotated poorly differentiated tumor regions (n = 25) with 2361.8560 m/z and 2528.8833 m/z showing statistical significance between the two groups. c Scatter plot comparison of Gal3Fuc2Man3GlcNAc6 and Gal3Fuc1Neu5Ac1Man3GlcNAc6 between all well differentiated (n = 40) and poorly differentiated (n = 25) tumor regions. Statistics performed between each group by multiple, unpaired t test with p < 0.05 considered significant. The following system was used to denote significance: p < 0.05 (*), p < 0.01 (**), p < 0.0001 (***), p < 0.00001 (****). Error bars correspond to standard deviation. d Receiver operator characteristic (ROC) curve demonstrating the predictive power (AUC = 0.77, p = 0.0003) of Gal3Fuc2Man3GlcNAc6 and Gal3Fuc1Neu5Ac1Man3GlcNAc6 loss for identifying poor differentiation of glands.