FIG. 7.

Differences in p53 modifications induced by IR and hypoxia. (A) IR but not hypoxia induces acetylation of Lys382. RKO cells were treated with hypoxia, 6 Gy of IR, or 20 μM ALLN for the times indicated. Immunoblotting was performed with a rabbit polyclonal antibody raised against Lys382. (B) Both IR and hypoxia induce Ser15 phosphorylation of p53. Treatment times are indicated. The top panel shows an immunoblot using a rabbit polyclonal that recognizes p53 phosphorylated at Ser15. The bottom panel shows the same immunoblot after being stripped and reprobed with the DO-1 monoclonal antibody.