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. 2023 Feb 24;13:22. doi: 10.1186/s13568-023-01521-2

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© The Author(s) 2023

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — Cytotoxicity evaluation of purified L-ASNase against cancer cell lines. a Cytotoxic effect of purified L-ASNase from PCH199. b E. coli L-ASNase (Elspar) on K562 blood cancer cell line using MTT assay. c The cytotoxic effect of L-ASNase was also tested towards normal cancer cell line IEC-6. d DAPI staining. Treated cells stained with DAPI clearly showed DNA fragmentation. Control cells showed uniform, rounded nuclei. L-ASNase-C indicates E. coli L-ASNase