Fig. 3.

a Yeast transformed with Htt 72Q, GFP showing the aggregation as foci. Fluorescence data show a reduction in aggregate when yeast model of HD was treated with vitamin B₆, B₁₂ and folate individually as well in combination (concentration used 20 μg/ml) when compared to control. Fluorescence quantification showing significant reduction of aggregation in treatment, t-test: *p-value ≤ 0.05. (n ≥ 10). b Treatment of B₆, B₁₂ and folate on 25Q and 72Q transformed yeast showed significant reduction in protein aggregates in 72Q, *p-value ≤ 0.05. c Filter retardation assay for yeast Htt 25Q/72Q and B₆, B₁₂ and folate treated samples. The intensity of the blot shows aggregation in control and its attenuation in B₆, B₁₂ and folate treated yeast samples. The intensity of the blot was calculated and shown to be significantly reduced in the treated sample with a *p-value < 0.05 and n = 3. d Transcriptomics analysis (present study) showing top 10 significantly deregulated pathways (adjusted p-value ≤ 0.05) in 72Q vitamin B₆, B₁₂ and folate treated sample and d expression of genes involved in these deregulated pathways (only genes with adjusted p-value ≤ 0.05) shown in form of a network. e Enrichment analysis showing deregulated pathways based on upregulated (shown in red) and downregulated genes (shown in blue) in 72Q B₆, B₁₂ and folate treated samples. f The pathway-gene interaction networks. Figure (f) was generated in Cytoscape 3.8.2. The plots d and e was made using Origin software