Figure 3.

PKA activation reduces cataracts induced by H₂O₂ in lens of WT, Cx50KO, and Cx46KO, but has no effect on Cx46/Cx50 dKO

(A–C) The lenses of WT, Cx50KO, Cx46KO, or dKO mice were kept transparent in culture media for 24 h with 5% CO₂ at 37°C before being treated with or without 0.5 mM H₂O₂ for 4 h, followed by treatment with 50 μM Forskolin or vehicle (DMSO) (left panel) or 2 mM 8-Br-cAMP or vehicle (dH₂O) as the control group (right panel). The opacity intensity of WT, Cx50KO, Cx46KO, and dKO in forskolin (B) or 8-Br-cAMP (C)-treated groups was measured by NIH ImageJ software. The data are presented as the mean ± SEM. (n ≥ 3). ∗, p < 0.05; ∗∗, p < 0.01; ∗∗∗, p < 0.001; ∗∗∗∗, p < 0.0001 (two-way ANOVA).