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. 2023 Feb 2;26(3):106114. doi: 10.1016/j.isci.2023.106114

Figure 5.

Figure 5

PKA activators increase PKA levels in the lens of WT, Cx50KO, Cx46 KO, and Cx46/Cx50 dKO in the absence or presence of H2O2 and UVB

(A–C) The lenses of WT, Cx50KO, Cx46KO, and dKO mice PKA levels were tested and quantified by Invitrogen PKA (Protein Kinase A) Colorimetric Activity Kit after lens were treated with or without 50 μM forskolin in the absence or presence of 0.5 mM H2O2. (One-way ANOVA) (B) PKA levels were tested and quantified after lens was treated with or without 2 mM 8-Br-cAMP in the absence or presence of 0.5 mM H2O2. (C) PKA levels were tested and quantified after the lens were treated with or without 50 μM forskolin in the absence or presence of 10 J/cm2 UVB radiation.

(D) PKA levels were tested and quantified after lens was treated with or without 2 mM 8-Br-cAMP in the absence or presence of 10J/cm2 UVB radiation. The data are presented as the mean ± SEM. (n ≥ 3). ∗, p < 0.05; ∗∗, p < 0.01; ∗∗∗, p < 0.001; ∗∗∗∗, p < 0.0001. (two-way ANOVA for B-D).