Figure 8.

PKA activation decreases ROS levels in WT, Cx50, and Cx46 KO, but not in Cx46/Cx50 dKO lens

(A–C) The lenses of WT, Cx50KO, Cx46KO, and dKO mice were kept transparent in culture media for 24 h at 37°C before being treated with 0.5 mM H₂O₂ (upper panel) or 10 J/cm² UVB (lower panel) for 4 h, and followed by treatment with 50 μM forskolin, or vehicle (DMSO) for 2 h. Intracellular ROS was measured by incubating lens with 10 μM Carbxyl-H2DCFDA for 30 min. Scale bar: 100 μm. H₂O₂-induced (B) or UVB-induced (C) intracellular ROS level was quantified by using NIH ImageJ software. The data are presented as the mean ± SEM. (n ≥ 3). ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001 (two-way ANOVA).