REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rabbit anti-SOD1	Santa Cruz Biotechnology, Dallas, Texas	Cat# sc-11407; RRID:AB_2193779
Rabbit anti-catalase	Santa Cruz Biotechnology, Dallas, Texas	Cat# sc-50508; RRID:AB_2275410
Monoclonal mouse anti-β-actin	Thermo Fisher Scientific	Cat# MA5-15739; RRID:AB_10979409
Monoclonal mouse anti-GAPDH	Thermo Fisher Scientific	Cat# AM4300; RRID:AB_2536381
Bacterial and virus strains
Recombinant RCAS(A) DNA constructs and high-titer retroviruses	Jiang and Goodenough, 199865	University of Texas Health Science Center at San Antonio
Cx50H156N	Banks et al., 200766	University of Texas Health Science Center at San Antonio
Biological samples
Fertilized white leghorn chicken eggs	Texas A&M Agriculture & Policy Science	Fertile egg
Chemicals, peptides, and recombinant proteins
Protease Inhibitor Cocktail	Thermo Fisher Scientific	Cat# 5892970001
Lucifer yellow	Thermo Fisher Scientific	Cat# L453
Rhodamine dextran	Thermo Fisher Scientific	Cat# T669
Dimethyl sulfoxide	Thermo Fisher Scientific	Cat# MT-25950CQC
Forskolin	Thermo Fisher Scientific	Cat# 34-427-010MG
Carboxy H2DCFDA	Thermo Fisher Scientific	Cat# C400
Paraformaldehyde	Thermo Fisher Scientific	Cat# 15710
Dulbecco’s modified Eagle’s medium (DMEM, high glucose)	Thermo Fisher Scientific	Cat# 11965118
Hydrogen peroxide, 30%	Thermo Fisher Scientific	Cat# H325-100
Chicken serum	Thermo Fisher Scientific	Cat# 16-110-082
TrypLE Express enzyme	Thermo Fisher Scientific	Cat# 12605–028
Medium 199	Thermo Fisher Scientific	Cat# 11-150-059
Phenol red-free medium 199	Thermo Fisher Scientific	Cat# 11-043-023
Penicillin-streptomycin	Thermo Fisher Scientific	Cat# MT30001CI
8-Br-cAMP	Thermo Fisher Scientific	Cat# NC1183334
Andwin ScientificTissue-Tek™ CRYO-OCT Compound	Thermo Fisher Scientific	Cat# 14-373-65
Thermo Scientific™ Pierce™ DAPI Nuclear Counterstain	Thermo Fisher Scientific	Cat# PI-62247
Critical commercial assays
Dead Cell Apoptosis Kit	Biolegend	Cat# 640914
PKA Colorimetric Activity Kit	Thermo Fisher Scientific	Cat# EIAPKA
Experimental models: Cell lines
Chicken embryonic fibroblast (CEF)	Laboratory of Dr. Jean Jiang	University of Texas Health Science center at San Antonio
Chick lens primary cell	Jiang et al., 199363	University of Texas Health Science center at San Antonio
Experimental models: Organisms/strains
Cx50 knockout (Cx50−/−) mouse; C57BL/6	White et al., 199822	Stony Brook University
Cx46 knockout (Cx46−/−) mouse; C57BL/6	White et al., 199822	Stony Brook University
Cx46 and Cx50 double knockout (Cx46−/−/Cx50−/−) mouse; C57BL/6	Gu et al., 201923	University of Texas Health Science center at San Antonio
Software and algorithms
Image Studio Lite	Image Studio Lite software	Image Studio Lite; RRID:SCR_013715
ImageJ	NIH ImageJ software	ImageJ; RRID: SCR_003070
GraphPad Prism 7 software	GraphPad software	GraphPad; RRID: SCR_000306