Skip to main content
. 2023 Feb 20;26:1–13. doi: 10.1016/j.bioactmat.2023.02.008

Fig. 5.

Fig. 5

In vitro proliferation and differentiation of BMSCs on different scaffolds. (A) Cell morphologies and attachment analysis of BMSCs on the surface of different scaffolds at 24 h. Green: cytoskeleton stained with FITC-Phalloidin, blue: cellular nuclei counterstained with DAPI. (B, C) The proliferation of BMSCs cultivated on the MBGS, CBP/MBGS and PTHrP-2-loaded scaffolds exposed to 808 nm laser irradiation (1 W/cm2, 10 min). (D) ALP staining of BMSCs cultured on different scaffolds at day 14. (E) Alizarin red staining of BMSCs mineral deposition on different scaffolds after 28 days. (F–H) Osteogenesis-related gene expressions of Col I (F), Runx2 (G), and BMP-2 (H) of BMSCs. ***P < 0.001,**P < 0.01, *P < 0.05.