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Combinations of PAGE-purified oligonucleotides annealed to produce branched DNA substrates
| DNA substrate | Fluorescently labeled oligonucleotide | Unlabeled oligonucleotides |
|---|---|---|
| Holliday junction | Oligo 1 (5′-FAM) | Oligos 2, 3, and 4 |
| 5′-flap | Oligo 1 (5′-FAM) | Oligos 4 and 2.5 |
| 3′-flap | Oligo 1 (5′-FAM) | Oligos 4 and 3.5 |
| Gapped dsDNA | Oligo 5 (5′-FAM) | Oligos 6 and 7 |
Oligonucleotide sequences are provided in the key resources table.
