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. 2023 Feb 20;4(1):102128. doi: 10.1016/j.xpro.2023.102128

2× Denaturing Loading Dye

Reagent Final concentration Amount
Formamide 90% (v/v) 9 mL
10× TBE 1 mL
Bromophenol blue 0.25% (w/v) 0.025 g
Xylene cyanol FF 0.25% (w/v) 0.025 g
Total 10 mL

Store the solution at room temperature (25°C). Use within 6 months.