2× Denaturing Loading Dye
| Reagent | Final concentration | Amount |
|---|---|---|
| Formamide | 90% (v/v) | 9 mL |
| 10× TBE | 1× | 1 mL |
| Bromophenol blue | 0.25% (w/v) | 0.025 g |
| Xylene cyanol FF | 0.25% (w/v) | 0.025 g |
| Total | 2× | 10 mL |
Store the solution at room temperature (25°C). Use within 6 months.