15% Denaturing Polyacrylamide Gel

Reagent	Final concentration	Amount
40% Acrylamide/Bis-acrylamide solution (19:1)	15%	37.5 mL
10× TBE	1×	10 mL
Urea	7 M	42 g
10% APS	0.09% (v/v)	900 μL
TEMED	0.075% (v/v)	75 μL
ddH2O	N/A	x mL to a total volume of 100 mL
Total	N/A	100 mL