Skip to main content
. 2023 Feb 8;26(3):106156. doi: 10.1016/j.isci.2023.106156

Figure 2.

Figure 2

Drug screening assay optimization and validation

(A and B) Effect of 250 nM Tasin-1 in MBP-NLuc expression at days 5, 10, and 15 presented as (A) raw RLU value and (B) RLU fold change (FC). The Z-factor score for FC is better than the raw RLU value. Data presented as mean and compared to DMSO using one-way ANOVA (∗∗∗, P <0.0001).

(C) Timeline of the drug screening assay used for our reporter cell system.

(D) Validation of our drug screening assay using the compounds known to enhance oligodendrocyte differentiation. Data presented as mean ±SEM.

(E) purified OPCs were plated on a plate containing electrospun nanofiber in the presence of DMSO or small molecules from (D). At least two of the compounds seems to increase the myelination of the nanofiber.