Ro1138412 and SR2211 promote differentiation of rodent OPCs
OPCs from P7-8 mice were purified using Anti-O4 microbeads and MACS purification method, and the purified OPCs were treated with one of the following molecules: Ro1138412, SR2211, T3 as positive control or DMSO.
(A) Immunostaining for MBP protein shows significant increase in MBP positive cells and MBP+ area in the drug treated samples.
(B–D) Quantification of MBP+ area (B) OLIG2+ cell number (C), and the MBP+ area relative to OLIG2+ cells in Ro1138412 treatment (top panels) and SR2211 treatment (bottom panels) compared to T3 and DMSO treatments. Data presented as mean ± SEM.