Figure 7.

Single cell transcriptomic analysis of the differentiating PtP1MsNL OPCs treated with different small molecules

(A) schematic of the scRNAseq experiment. Day 80 differentiating OPCs were treated with small molecules for ten days before dissociation, single cell capture, and sequencing.

(B) Unsupervised clustering of the single cell transcriptional profiles of combined dataset including all the DMSO and drug treated samples. Cells are visualized with an UMAP embedding. The unbiased clustering divided the cell into 15 clusters.

(C) UMAP projection to visualize overlay of cells with different drug treatments.

(D) Trajectory analysis illustrating the maturation of mitotic progenitor cells to OL and astrocytes. Astrocytes seem to originate from early progenitors as well as OPCs.

(E) A dot plot showing average expression of a subset of known marker genes (Xaxis) across different clusters (Yaxis).

(F) heatmap of the top 10 enriched genes from each cluster. Yellow color represents increased expression.

(G and I) Proportion of cells in the oligodendrocyte and pre-oligodendrocytes clusters (G) and astrocyte clusters (I) between different drug treated samples.

(H) Average expression of oligodendrocyte markers genes in the OL cluster. Data in (G-I) are presented as mean ±  SEM