REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-PDGFR alpha	R&D Systems	AF-307-SP
PE anti-human CD140a (PDGFRalpha)	BioLegend	323505
Anti-SOX10	R&D Systems	AF2864-SP
Anti-NKX2.2	DSHB	Cat# 74.5A5; RRID:AB_531794
Anti-MBP	Millipore	Cat# MAB386; RRID:AB_94975
Anti-OLIG2	Millipore	Cat# AB9610, RRID:AB_570666
Anti-O4 clone 81	Millipore	MAB345
Hoechst 33342	ThermoFisher Scientific	H3570
Alexa Fluor 488 goat anti-rabbit IgG	ThermoFisher Scientific	A-11034
Alexa Fluor 488 goat anti-mouse IgM	ThermoFisher Scientific	A-21042
Alexa Fluor 647 goat anti-rabbit IgG	ThermoFisher Scientific	A-21245
Alexa Fluor 647 donkey anti-goat IgG	ThermoFisher Scientific	A-21447
Bacterial and virus strains
DH10B	ThermoFisher Scientific	C404010
Chemicals, peptides, and recombinant proteins
mTeSR1	Stem Cell Technologies	5850
StemFlex	ThermoFisher Scientific	A3349401
DMEM/F12	ThermoFisher Scientific	11320082
Accutase	ThermoFisher Scientific	A11105-01
Antibiotic-Antimycotic	ThermoFisher Scientific	15240062
Growth Factor Reduced BD MatrigelTM Matrix	BD Biosciences	354230
Blebbistatin	Sigma	B0560-1MG
Opti-MEM™ I Reduced Serum Medium	ThermoFisher Scientific	31985088
CryoStor® CS10	Stem Cell Technologies	7930
CTLC-ABC	Immunospot	NC9711917
Lipofectamine™ Stem Transfection Reagent	ThermoFisher Scientific	STEM00001
Puromycin	ThermoFisher Scientific	A11138-03
N2 supplement	ThermoFisher Scientific	17502-048
B27 Supplement without vitamin A	ThermoFisher Scientific	12587-010
SB431542	Stem Cell Technologies	72234
LDN193189	Stem Cell Technologies	72147
Smoothened agonist	Sigma	R2625
All-trans retinoic acid	Millipore	566660
Recombinant human PDGF-AA	R&D Systems	221-AA-050
Recombinant human IGF-I	R&D Systems	291-G1-200
Recombinant human HGF	R&D Systems	294-HG-025
Neurotrophin 3 (NT3)	Millipore	GF031
cAMP analog	Sigma	D0260
3,3,5-Triiodo-l-thyronine (T3)	Sigma	T2877
l-Ascorbic acid	Sigma	A4403
Insulin solution human	Sigma	19278
Biotin	Sigma	4639
Poly-L-ornithine hydrobromide	Sigma	3655
HEPES (1 M)	ThermoFisher Scientific	15630080
Laminin	MilliporeSigma	L2020-1MG
Critical commercial assays
MycoAlert Mycoplasma Detection kit	Lonza	LT07-218
hPSC Genetic Analysis Kit	StemCell Technologies	7550
Nano-Glo® Luciferase Assay System	Promega	N1120
Neural Tissue Dissociation Kit	Miltenyi	130-092-628
Deposited data
Single cell RNAseq	This study	Database: GSE223599
Human single cell data	Chamling et al.,13 Jakel et al.29	GEO Database: (GSE118257, GSE104276GSE146373), Bioproject (544731))
Human reference genome NCBI build 37, GRCh37	Genome Reference Consortium	http://www.ncbi.nlm.nih.gov/projects/genome/assembly/grc/human/
Experimental models: Cell lines
Passage 40 hESC (WA09) or H9 NIH HES # 0062	WiCell	RRID:CVCL_9773
Passage 26 hESC (RUES1)	WiCell	RRID:CVCL_B809
PTt-P1-MsNL (the triple reporter)	This paper	N/A
Oligonucleotides
Primers for Reporter Cell line (see Table S8)	This paper	N/A
qPCR Primers (see Table S8)	This paper	N/A
Recombinant DNA
PX459.V2	Addgene	RRID:Addgene_62988
ZeroBlunt TOPO	ThermoFisher Scientific	K280020
MBP-P2A-secNanoluc	This paper	N/A
PLP1-sfGFP	This paper	N/A
Software and algorithms
ImageJ	Schneider et al.52	https://imagej.net/software/fiji/
GraphPad Prism, V9	GraphPad Software, LLC	https://www.graphpad.com
CFX Maestro	Biorad	12004128
Harmony software 4.9	Perkin Elmer	HH17000010
Cellomics CX7	ThermoFisher Scientific	HCSDCX7LEDPRO
R package	Wu et al.53	ClusterProfiler