Table 1.
Primers targeting the polymerase basic 1 (PB1) gene segment for a pan-influenza hemi-nested RT-PCR assay.
| Primer | Sequence 5′-3’ |
|---|---|
| First round RT-PCR | Expected amplicon size: 699 bp |
| orthoA PB1 936 F1 | GGR GAC AAY ACM AAR TGG AAT G |
| orthoA PB1 1500 R1/2 | GTT KAT CAT RTT GKT YTT KAT YAC TG |
| Hemi-nested RT-PCR | Expected amplicon size: 396 bp |
| orthoA PB1 1200 F2 | CCW GGR ATG ATG ATG GGN ATG TTC |
Primers are named according to the virus target, genome segment, approximate position in the reference genome (H1N1, accession: NC_026435.1), and primer orientation. Degenerate nucleotides are as follows: R = A/G, Y = T/C, M = A/C, K = T/G, W = A/T, N = any nucleotide.