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Probes and primer sequences of multiplex quantitative PCR assays.
| Gene | Probes and Sequences of Primers (5′ → 3′) | Amplicon Lengths (bp) |
|---|---|---|
| Human beta-2-microglobulin (M17987) | Forward: GCT GGG TAG CTC TAA ACA ATG TAT TCA | 94 |
| Reverse: CCA TGT ACT AAC AAA TGT CTA AAA TGGT | ||
| Probe: VIC-CAGCAGCCTATTCTGC | ||
| Human mitochondrial (NC-012920) | Forward: CTT CTG GCC ACA GCA CTT AAA C | 65 |
| Reverse: GCT GGT GTT AGG GTT CTT TGTTT | ||
| Probe: FAM-ATCTCTGCCAAACCCC |
