Table 3.

Detection of DWV-A and DWV-B in workers of Apis florea and A. mellifera by RT-PCR.

Sample Source	Sample Size (Af/Am)	Location	A. florea (Af) *			A. mellifera (Am) *
			DWV-A		DWV-B	DWV-A		DWV-B
			DWV	KV		DWV	KV
OPEN SPACE
	5/3	Qianzhen	negative	positive	negative	negative	negative	negative
	5/3	Qianzhen	positive	positive	negative	negative	negative	negative
	6/2	Qianzhen	negative	positive	negative	negative	negative	negative
	5/0	Qianzhen	negative	positive	negative
	5/2	Gushan	negative	positive	negative	negative	positive	negative
	5/0	Gushan	negative	positive	negative
	4/4	Fengshan	negative	positive	negative	negative	positive	negative
	5/4	Fengshan	negative	positive	negative	negative	positive	negative
	8/0	Fengshan	negative	positive	negative
	8/1	Qijin	negative	positive	negative	negative	negative	negative
	14/2	Qijin	negative	positive	negative	positive	negative	negative
	4/4	Gangshan	negative	positive	negative	negative	positive	negative
	4/3	Lingya	negative	positive	negative	negative	negative	negative
NEST
	4/0	Xiaogang	positive	positive	negative
	6/0	Xiaogang	negative	positive	negative
	4/0	Qianzhen	positive	positive	negative
	4/0	Qianzhen	positive	positive	negative
	4/0	Qianzhen	positive	positive	negative
	5/0	Gushan	negative	positive	negative
	7/0	Gushan	negative	positive	negative
	6/0	Lingya	negative	positive	negative
	6/0	Lingya	negative	positive	negative
	8/0	Lingya	positive	positive	negative
	8/0	Fengshan	negative	positive	negative
	6/0	Qijin	negative	positive	negative

* positive: DWV-A or DWV-B detected by PCR in worker samples; negative: DWV-A or DWV-B not detected by PCR in worker samples; primer use: DWV-F/-R [14], KV-F/-R [7].