TABLE 1.
Liver weights and serum chemistries of conditionally HNF4α-null mice and controlsa
| Parameter | Value for indicated mouse type
|
|||
|---|---|---|---|---|
| WT | AlbCre | H4LivKO | H4Flox | |
| Liver | ||||
| Wet wt (g) | 0.9 ± 0.1 | 0.9 ± 0.2 | 1.3 ± 0.3∗∗ | 0.8 ± 0.2 |
| % body wt | 4.0 ± 0.3 | 4.0 ± 0.2 | 7.3 ± 0.9∗∗ | 3.9 ± 0.1 |
| Serum chemistry | ||||
| Albumin (mg/dl) | 3.8 ± 0.4 | 3.8 ± 0.3 | 4.0 ± 0.4 | 4.0 ± 0.3 |
| ALT (U/liter) | 40 ± 20 | 40 ± 23 | 65 ± 7∗ | 40 ± 20 |
| Triglycerides (mg/dl) | 86 ± 23 | 76 ± 20 | 38 ± 7∗∗ | 80 ± 22 |
| Total cholesterol (mg/dl) | 119 ± 15 | 114 ± 14 | 48 ± 15∗∗ | 121 ± 28 |
| HDL cholesterol (mg/dl) | 93 ± 25 | 88 ± 22 | 37 ± 23∗∗ | 104 ± 24 |
| Free fatty acids (mg/dl) | 1.3 ± 0.4 | 1.3 ± 0.4 | 1.3 ± 0.2 | 1.8 ± 0.9 |
| Bile acids (μmol/liter) | 16 ± 7 | 17 ± 11 | 283 ± 61∗∗ | 10 ± 7 |
| Glucose (mg/dl) | 150 ± 32 | 145 ± 28 | 97 ± 39 | 127 ± 36 |
a
Mice (45 days old) were fasted for 4 h, anesthetized, and decapitated, and serum was prepared. All values are means ± standard deviations from at least four animals (not separated by sex). Statistics were performed using one-way analysis of variance followed by an unpaired Student t test if applicable. ∗, P < 0.05; ∗∗, P < 0.01. ALT, alanine aminotransferase; WT, wild type.