Figure 6.

BN-PAGE analyses of membrane protein change kinetics of the lysogenic bacterium grown in human serum. T: TSBYE broth; H: TSBYE broth with 50% human serum; Numbers (0, 3, 6, 9, and 12) represent hours of incubation. Protein bands at two positions, B₁ at approximately the 1.5 mDa position and B₂ at the position of ~700 kDa, were detected from the lysogen with particularly strong signals after 6 h exposure in human serum. Four excised gel samples at the B₁ position prepared as B₁_T₀/H_o, B₁_H₃, B₁_H₆, and B₁_H₉, and five samples at the B₂ position prepared as B₂_T₀/H_o, B₂_H₃, B₂_H₆, B₂_H₉, and B2_H₁₂, were collected for semi-quantitative LC/MS analysis and data are described in Table 3 and Table 4.