Skip to main content
. 2023 Feb 7;24(4):3308. doi: 10.3390/ijms24043308

Figure 5.

Figure 5

NumbL interaction with TRAF6 was increased under GLT conditions. (A) Representative blot of TRAF6 proteins pulled down using an anti-NumbL antibody. (B) Representative blot of TRAF6 protein and quantification of TRAF6 protein levels in cells treated with GLT for the indicated time periods. (C) Representative immunoblot from co-immunoprecipitation experiments confirming the interaction between NumbL, TRAF6, and Mig6 under GLT conditions. (D,E) Effect of TRAF6 inhibitor 6877002 on the levels of cleaved caspase-3 and IkBa proteins in beta cells exposed to GLT for 12 h. (F) Proposed model of interactions between Mig6, NumbL, and TRAF6 on EGFR and NFkB signaling in beta cells. * p < 0.05 vs. DMSO control under NG conditions and # p < 0.05 vs. DMSO control under GLT conditions. The data are representative from at least three independent experiments.