Figure 1.

LentiMPRA analysis pipeline. (A) Selection of the 7200 candidate regulatory sequences (CRS) generated from the reference and alternate allele sequences of 3600 variants. (B) Selection process of the top active regions in N2 cells used to generate the positive control sequences. (C) Selection process for generating the negative control sequences. (D) Diagram of the lentiviral vector used for infection into our three replicate NPC cell populations. (E) Diagram of the computational pipeline used to associate and count the randomly added CRS and barcode sequences to be used for differential expression analysis [7].