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. 2023 Jan 27;15(2):360. doi: 10.3390/v15020360

Figure 6.

Figure 6

Overexpression of ADAM10 and ADAM17 increases the generation of S2′ and S2″ products but decreases cell-to-cell fusion. (A) HeLa cells were transiently co-expressed with double-tagged spike protein (N-terminal HA-tag; C-terminal V5-tag), WT (S), and ACE2 alone or in combination with empty vector (EV), ADAM10 or ADAM17. The V5 immunoblot shows the analysis of cell extracts 28 h post transfection. Note that we had to overexpose the gel to better see S2′. (B) Donor HeLa cells expressing empty vector (EV) or spike-glycoprotein WT (S) were co-cultured with acceptor TZM-bl cells expressing ACE2 and/or ADAM10 and ADAM17. The extent of fusion is represented as relative luminescence units (RLU). Representative blots of at least three independent experiments are shown. p values (**, p < 0.01; ****, p < 0.0001) were evaluated by a Student’s t-test.