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. 2023 Feb 13;24(4):3747. doi: 10.3390/ijms24043747

Figure 3.

Figure 3

ERβ1 and ERβ2 inversely affect the antiproliferative efficacy of ATRA. (A) Proliferation of MCF7-WT, MCF7-ERβ1 and MCF7-ERβ2 cells treated with vehicle (0.1% DMSO) or increasing concentrations of ATRA for six days. Relative cell numbers were expressed as percentage of cell number of vehicle-treated cells. Plots are representative of at least three independent experiments carried out in triplicate. (B,C) Proliferation of MCF7-WT, MCF7-ERβ1 and MCF7-ERβ2 cells treated with vehicle (0.1% DMSO), OHT (1 nM), ICI (1 nM), ATRA (100 nM) or their combination, as indicated, for six days. Relative cell numbers were expressed as percentage of cell number in vehicle-treated cells. Values (mean ± SEM) are from at least three independent experiments carried out in triplicate. * p < 0.05 vs. similarly treated MCF7-WT, # p < 0.05 vs. respective OHT- or ICI-treated cell line (t-test). OHT, 4-hydroxytamoxifen; ATRA, all-trans retinoic acid; ICI, ICI182,780.