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. 2023 Feb 11;15(2):500. doi: 10.3390/v15020500

Figure 2.

Figure 2

Identification of recombinant NDV expressing the gB protein of ILTV. (A) The insertion of the gB gene into rTS09-C was confirmed by RT-PCR using a pair of primers spanning the insertion region of the ILTV-gB gene. (B) BHK-21 cells were infected with rTS-gB or rTS09-C at an MOI of 0.1. The cells were harvested and lysed at 48 hpi, and the cell lysates were analyzed by western blotting using anti-gB or anti-NDV antibodies. (C) BHK-21 cells were infected with rTS-gB or rTS09-C at an MOI of 0.1. At 48 hpi, the cells were subjected to indirect immunofluorescence staining using anti-gB or anti-NDV antibodies. The cells were analyzed by confocal microscopy using ZEN 2.3 Lite software (Zeiss, Germany).