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. 2023 Feb 5;24(4):3142. doi: 10.3390/ijms24043142

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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The accuracy of the tomato CrRLK1L annotations in NCBI was higher than that in SGN. (a) The different versions of the gene structure and protein domain of SlCrRLK1L20 in the NCBI and SGN databases. The data were extracted from NCBI and SGN and then analyzed for visualization; detailed information can be found in Table S2. (b) Polypeptide antigen location in SlCrRLK1L20^NCBI and SlCrRLK1L20^SGN. (c) Amplification of the SlCrRLK1L20^NCBI and SlCrRLK1L20^SGN CDSs. (d) Western blot analysis of SlCrRLK1L20^NCBI and SlCrRLK1L20^SGN. 1: N. benthamiana leaves transiently expressing CaMV35S::SlCrRLK1L20^NCBI-HA; 2: N. benthamiana leaves transiently expressing CaMV35S::SlCrRLK1L20^SGN-HA; 3: S. lycopersicum fruit. The uncropped Western blot gel image can be found in Figure S2. (e) Confocal analysis of SlCrRLK1L20^NCBI and SlCrRLK1L20^SGN subcellular localization. Bars = 50 μm.