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Steady-state kinetic analysis of hGSTA1-1 and its mutants using GSH as a variable substrate (I) and CDNB at a fixed concentration. Steady-state kinetic analysis of hGSTA1-1 and its mutants using the CDNB as a variable substrate (II) and GSH at a fixed concentration. (A) hGSTA1-1, (B) hGSTA1-K141H, (C) hGSTA1-K141H/S142H, (D) hGSTA1-E137H, and (E) hGSTA1-E137H/K141H. Experiments were performed in triplicate. The inside graphs are the Lineweaver-Burk plots.
