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. 2023 Feb 12;24(4):3700. doi: 10.3390/ijms24043700

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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(A) Enzyme residual activity curves for hGSTA1-1 (black line), hGSTA1-K141H (grey line), hGSTA1-K141H/S142H (red line), hGSTA1-E137H/K141H (green line), and hGSTA1-E137H (orange line). The residual activities were measured after heat treatment at various temperatures (°C) for 5 min. Values presented are given as a mean of triplicates ± SE. (B) Melting curve measurements by DSF (scan rate of 1 °C/min) for hGSTA1-1(black), hGSTA1-K141H (blue), hGSTA1-K141H/S142H (red), hGSTA1-E137H/K141H (green), and hGSTA1-E137H (light green).