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. 2023 Feb 12;24(4):3700. doi: 10.3390/ijms24043700

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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(A) Screening of inhibition potency of selected pesticides (25 μΜ) against the wild-type enzyme and its mutants. The inhibition was evaluated by measuring GST catalytic activity using GSH-CDNB as substrate system, in the presence of 25 μΜ of pesticide for a period of 2 min. In the absence of pesticides, enzyme activity was taken as 100%. Experiments were performed in triplicate, and the reported values in the heat map correspond to the mean value. (B) Concentration–response curve for endosulfan. IC50 value for hGSTA1-K141H/S142H was determined using GraphPad Prism. GST activity was assayed using the CDNB–GSH assay system in triplicate (standard errors are depicted).