Table 2.

Species-, genus-, and group-specific primers targeting the 16S rRNA and qPCR characteristics of gut microbiota analysis.

Target	Primer	Primer Sequence (5′-3′)	Reference Strains	References
Total Bacteria	Forward	TCCTACGGGAGGCAGCAGT	Bacteroides fragilis MM44 (ATCC 25285)	[28]
Total Bacteria	Reverse	GGACTACCAGGGTATCTAATCC TGTT	Bacteroides fragilis MM44 (ATCC 25285)	[28]
Lactobacillus group	Forward	AGCAGTAGGGAATCTTCCA	L. gasseri DSM 20243	[29]
Lactobacillus group	Reverse	CACCGCTACACATGGAG	L. gasseri DSM 20243	[29]
Bifidobacterium spp.	Forward	TCGCGTCYGGTGTGAAAG	B. bifidum DSM 20456	[29]
Bifidobacterium spp.	Reverse	CCACATCCAGCRTCCAC	B. bifidum DSM 20456	[29]
F. prausnitzii	FPR-2F	GGAGGAAGAAGGTCTTCGG	F. prausnitzii DSM 17677	[30,31,32]
F. prausnitzii	Fprau645R	AATTCCGCCTACCTCTGCACT	F. prausnitzii DSM 17677	[30,31,32]
A. muciniphila	AM1	CAGCACGTGAAGGTGGGGAC	A. muciniphila DSM 22959	[33,34]
A. muciniphila	AM2	CCTTGCGGTTGGCTTCAGAT	A. muciniphila DSM 22959	[33,34]
C. perfringens group	CPF	ATGCAAGTCGAGCGATG	C. perfringens ATCC 13124	[35]
C. perfringens group	CPR	TATGCGGTATTAATCTCCCTTT	C. perfringens ATCC 13124	[35]
Prevotella spp.	g-Prevo-F	CACRGTAAACGATGGATGC	Prevotella copri DSM 18205	[36]
Prevotella spp.	g-Prevo-R	GGTCGGGTTGCAGACC	Prevotella copri DSM 18205	[36]
Bacteroides spp.	Bac303F	GAAGGTCCCCCACATTG	B. fragilis MM44 (ATCC 25285)	[37]
Bacteroides spp.	Bfr-Fmrev	CGCKACTTGGCTGGTTCAG	B. fragilis MM44 (ATCC 25285)	[37]