Figure 2.

Effects of inactivation of MKS1 in wild-type and rtg2Δ cells on Rtg1p and Rtg3p. (A) Subcellular localization of C-terminal GFP derivatives of Rtg1p and Rtg3p in ρ⁺ wild-type (WT) and mutant derivatives of strain PSY142. Constructs encoding the GFP derivatives were transplaced into the respective RTG1 and RTG3 loci. (B) Rtg3p is dephosphorylated in mks1Δ cells grown in YNBD + cas medium. Rtg3p was detected by Western blotting with a polyclonal anti-Rtg3p antibody raised against recombinant Rtg3p (Sekito et al., 2000). Lane 2 shows that the mobility of Rtg3p collapses to a faster migrating species when an extract of wild-type cells was pretreated with 5 U of calf intestinal phosphatase (CIP).