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. 2023 Feb 14;12(4):860. doi: 10.3390/plants12040860

Figure 2.

Figure 2

The effect of Ca-EE on β-hexosaminidase release in IgE- and HSA-treated RBL-2H3 cells. (a) RBL-2H3 cells were incubated with DNP-IgE and DNP-HSA to induce β-hexosaminidase activity. Cells were treated with Ca-EE at the indicated concentration. The supernatant of the cell culture was transferred to a plate and reacted with glucosaminide for 2 h. β-Hexosaminidase release was examined by measuring absorbance at 405 nm. (b) Cell viability of RBL-2H3 cells under Ca-EE treatment (0–100 μg/mL). Six replicates were set in these assays (a,b). The conventional MTT assay was performed ## p < 0.01 compared with the normal group; ** p < 0.01 compared with the control group.