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. 2023 Feb 14;12(4):860. doi: 10.3390/plants12040860

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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In vivo efficacy of Ca-EE in an atopic dermatitis (AD) mouse model. (a) Schematic diagram of the AD mouse disease model. Ca-EE was topically applied to skin once a day for 7 days before DNCB treatment. AD was induced by DNCB treatment for an additional 21 days. (b) Ears of AD-induced mice. Photographs were taken at day 28. (c) The dermatitis score was determined by changes of itching, erythema, and dryness of lesion (Criteria 0: no change, 2: slight, 4: moderate, 8: severe change). (d) The ear thickness of mice was measured by dial thickness gauges. (e) The body weight of mice was measured every 3 days. (f) The water content (%) of skin was evaluated using an SK-IV digital moisture monitor. ^## p < 0.01 compared with the normal group; ** p < 0.01 compared with the control group.