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. 2023 Feb 14;12(4):860. doi: 10.3390/plants12040860

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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In vivo effect of Ca-EE in the DNBC-induced AD mouse model. (a,b) In DNBC-induced AD disease model mice, ears and blood were collected for hematological analysis and stained with hematoxylin and eosin (H&E) (a) and toluidine blue O (b). (c) The numbers of toluidine blue O-stained mast cells were counted with a cell counter. (d) Serum was isolated, and the levels of IgE were examined by ELISA. (e) The mRNA level of IL-4 in ear lysates was detected by real-time quantitative PCR. ^## p < 0.01 compared with the normal group; ** p < 0.01 compared with the control group.

In vivo effect of Ca-EE in the DNBC-induced AD mouse model. (a,b) In DNBC-induced AD disease model mice, ears and blood were collected for hematological analysis and stained with hematoxylin and eosin (H&E) (a) and toluidine blue O (b). (c) The numbers of toluidine blue O-stained mast cells were counted with a cell counter. (d) Serum was isolated, and the levels of IgE were examined by ELISA. (e) The mRNA level of IL-4 in ear lysates was detected by real-time quantitative PCR. ^## p < 0.01 compared with the normal group; ** p < 0.01 compared with the control group.