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. 2023 Feb 14;16(2):298. doi: 10.3390/ph16020298

Figure 2.

Figure 2

Treatment of Alzheimer’s astrocytes at various 4-PBA concentrations in conditioned medium. (A) WT and 3Tg-iAstro were treated with astrocyte conditioned medium (ACM), astrocyte conditioned medium with 4-PBA 3 µM freshly added (ACM + fresh 4-PBA), or astrocyte conditioned medium collected form astrocytes treated with 4-PBA for 48 h (ACM + 4-PBA 48 h). After 48 h, cells were pulsed with 4 µM puromycin and lysate for 1 h, then protein synthesis rate was accessed via WB. Concentrations of 4-PBA were assessed by LC-HRMS. (B) Representative WB of protein synthesis analysis, (C) data are expressed as mean ± SEM of 3 independent experiments, (D) 4-PBA absorption determined by LC-HRMS; concentrations are expressed as µM ± SEM of 3 independent experiments repeated twice. (* p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001; by one-way ANOVA, Sidak’s multiple comparison.).