Table 2.
Proteins (n = 9; signal > 1000 ppm; ≥ 3-fold vs. planktonic growth; p < 0.01, t-test) significantly upregulated in biofilm-grown S. maltophilia Sm126 cells.
| Spot No. | Protein | Biological Process | Molecular Function |
Fold Up-Regulation a | Peptides Matched b | Sequence Coverage c | pI d | MW e | GI f |
|---|---|---|---|---|---|---|---|---|---|
| MB4713 | Putative urocanate hydratase | Aminoacid degradation |
Conversion of urocanate to 4-imidazolone-5-propionate | 7.8 | 13 | 30 | 5.58/6.06 | 60,368/69,065 | 190575003 |
| MB4807 | Putative zinc metallopeptidase |
Cell division; Protein catabolysis; Proteolysis |
Metallopeptidase activity; Zinc ion binding |
10.9 | 15 | 25 | 5.73/6.05 | 76,512/82,630 | 190572608 |
| MB1806 | Putative 30S ribosomal protein | Protein synthesis | Protein synthesis | 3.0 | 4.44/4.79 | 66,234/72,144 | 1702195087 | ||
| MB6403 | Putative aminotransferase |
Protein metabolism; Gluconeogenesis | Transamination | 4.7 | 7 | 31 | 6.07/6.91 | 42,450/43,319 | 190575152 |
| MB6405 | Putative NADP-dependent alcohol dehydrogenase | Response to reactive oxygen species | Metal ion binding; Butanol dehydrogenase activity | 4.3 | 4 | 17 | 5.71/6.93 | 38,658/41,493 | 190575829 |
| MB7303 | Putative threonine 3-dehydrogenase | Threonine catabolism |
L-threonine 3-dehydrogenase activity | 3.5 | 20 | 47 | 6.21/7.26 | 37,502/35,401 | 190572994 |
| MB7502 | Putative serine hydroxymethyltransferase | Glycine biosynthesis from serine |
Glycine hydroxymethyltransferase activity | 10.1 | 9 | 27 | 6.12/7.44 | 45,122/49,476 | 190572766 |
| MB8406 | Conserved hypothetical protein |
Not known | Not known | 7.8 | 22 | 59 | 7.15/5.99 | 43,882/85,923 | 190576469 |
| MB8602 | Putative biotin carboxylase |
Fatty acid biosynthesis |
ATP binding; Metal ion binding |
5.2 | 13 | 34 | 6.55/7.97 | 49,609/60,821 | 190576068 |
a Ratio of mean percentage integrated optical density for each protein from biofilm-grown and planktonic S. maltophilia cells (n = 6). b Number of tryptic peptides matched to protein sequence based on MS/MS spectra. c The percentage of amino acid coverage (peptides observed/theoretical number from sequence data given in S. maltophilia database). d Theoretical and practical pI of the protein. e Theoretical and practical mass, expressed in Da, of the protein. f GI accession (GenInfo identifier; sequence identification).