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. 2002 Mar;13(3):817–829. doi: 10.1091/mbc.01-08-0402

Figure 1.

Figure 1

Identification and molecular analysis of human D2LIC. (A) Silver-stained gel of sucrose gradient fractions and immunoprecipitated complexes. COS-7 cell homogenate was fractionated on sucrose gradients and 15 S peak fractions pooled (lane 1). Immunoprecipitations from the 15 S fractions were performed using preimmune serum (lane 2) and DHC2 affinity-purified antibodies (lane 3). An ∼33-kDa polypeptide (arrow) that coimmunoprecipitated with DHC2 was excised from the gel and microsequenced. (B) DNA and predicted protein sequence for the human D2LIC. Two peptide fragments generated from microsequencing (underlined) were used to identify a clone (D2LIC) from the human dbEST database. The P-loop consensus motif is underlined and in bold. (C) Northern blot analysis. COS-7 poly(A+) RNA (lane 1), total COS-7 RNA (lane 2), and total HeLa RNA (lane 3) were size fractionated on a gel, transferred to nylon, and probed with the full-length D2LIC clone (shown in B). A predominant 1.6-kb message is evident.