Figure 7.

Effect of brefeldin A on the localization of D2LIC and DHC2 to the Golgi. COS-7 cells were treated at 37°C with 0.4% ethanol (A, D, and G), 10 μg/ml BFA dissolved in ethanol (B, E, H, and J–L) for 30 min, or 10 μg/ml BFA for 30 min followed by recovery in the absence of BFA for 90 min (C, F, and I). After these treatments, cells were fixed and double labeled with anti-D2LIC (A–C) and anti-DHC2 (D–F) or stained with anti-p58 (G–I). DNA in all cells is visualized with DAPI (shown in blue). BFA-treated cells were also double labeled with anti-D2LIC (J) and anti-γ-tubulin (K). The corresponding merged image is shown in L. Bars, 10 μm.