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. 2023 Jan 19;15(2):340. doi: 10.3390/pharmaceutics15020340

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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In vitro assay for determination of relative scratch-wound recovery of HaCaT cells incubated with 1% atraric acid (AA) topical formulations (AA–TF#1 and AA–TF#15), 1% minoxidil in ethanol (EtOH), or vehicles of AA–TF#1 or #15 diluted to a drug concentration equivalent to 50 µM: (a) Time-course curve of relative scratch-wound recovery area of HaCaT cell monolayers. ** p < 0.01 compared to control (0.5% FBS); ^### p < 0.001 compared to vehicle (AA–TF#1); ^$ p < 0.05, ^$$$ p < 0.001 compared to vehicle (AA–TF#15). Values represent mean ± SD (n = 4); (b) Representative microscope images of scratch-wounds of HaCaT cells. Scale bar = 200 µm.