Table 2.

ATPase analysis of drNurA-HerA and mutant

HerAb	NurAb	ATPase mol/protein/min (no DNA)	ATPase mol/protein/min (ss DNA)	ATPase mol/protein/min (ds DNA)
wta	-	<<0.01	<<0.01	<<0.01
-	wta	<<0.01	<<0.01	<<0.01
wta	wta	0.08±0.03	0.52±0.09	0.45±0.07
wt	wt	0.42±0.23	28.63±1.78	4.18±0.36
wt	-	<<0.01	1.69±0.09	0.09±0.04
-	wt	<<0.01	<<0.01	<<0.01
HerAm	wt	<<0.01	6.01±1.54	0.38±0.09
487/8A	wt	<<0.01	<<0.01	<<0.01
495A	wt	0.46±0.15	6.53±1.11	0.72±0.14
552A	wt	0.39±0.21	9.68±1.81	0.86±0.19
96A	wt	0.45±0.17	27.93±1.99	4.01±0.46
wt	53A	0.43±0.09	24.23±2.38	3.04±0.45
wt	201A	0.46±0.13	21.11±1.98	3.81±0.39
wt	309A	0.34±0.12	28.31±2.71	2.98±0.51
wt	dPin	0.27±0.02	15.85±2.04	0.10±0.04
wt	8A	0.36±0.16	26.64±1.76	0.28±0.08
wt	13A	0.39±0.11	27.13±1.01	3.94±0.19

^aindicate Mg²⁺ was used instead of Mn²⁺ in this assay

^b indicate the addition of wild type or mutated proteins

The measurements were repeated more than six times